C18zip Online

Dispense the liquid into a clean, sterile microcentrifuge tube.

Repeat . This prepares the resin to bind the peptides. 4. Sample Binding

50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste. C18zip

The sample is now ready for (MALDI-TOF or LC-MS/MS).

Repeat using the same elution volume to ensure all peptides are recovered. Dispense the liquid into a clean, sterile microcentrifuge

Peptides dissolved in 0.1% Trifluoroacetic acid (TFA). Wetting Solution: 100% Acetonitrile (ACN). Equilibration/Wash Solution: 0.1% TFA in water.

💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry. Wetting the Tip Set your pipette to 10 µL

Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials